Characterization of Transport and Adsorption Mechanisms in Chromatographic Media
Abstract:
This work deals with experimentally determined binding orientations of lysozyme on different chromatographic adsorber materials at varying mobile phase compositions (ionic strength and pH). Findings were correlated with molecular dynamics simulations and used to obtain a model approach to predict protein retention times in ion-exchange chromatography. The second part of this work deals with confocal laser-scanning microscopy as a tool to visualize protein transpiort in chromatographic media.
| Zugehörige Institution(en) am KIT | Institut für Bio- und Lebensmitteltechnik (BLT) |
| Publikationstyp | Hochschulschrift |
| Publikationsjahr | 2009 |
| Sprache | Englisch |
| Identifikator | urn:nbn:de:swb:90-158163 KITopen-ID: 1000015816 |
| Verlag | Universität Karlsruhe (TH) |
| Art der Arbeit | Dissertation |
| Fakultät | Fakultät für Chemieingenieurwesen und Verfahrenstechnik (CIW) |
| Institut | Institut für Bio- und Lebensmitteltechnik (BLT) |
| Prüfungsdaten | 18.12.2009 |
| Schlagwörter | ion-exchange chromatography, protein adsorption, binding mechanism, pH and ionic strength influence, molecular dynamic simulations |
| Referent/Betreuer | Hubbuch, J. |