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Elevated UV-B radiation reduces genome stability in plants

Ries, G.; Heller, W.; Puchta, H.; Sandermann, H.J.; Seidlitz, H.K.; Hohn, B.

Abstract:
Long-term depletion of the stratospheric ozone layer contributes to an increase in terrestrial solar ultraviolet-B radiation$^{1,2,3}$. This has deleterious effects on living organisms, such as DNA damage$^{4,5}$. When exposed to elevated ultraviolet-B radiation (UV-B; 280–315 nm), plants display a wide variety of physiological and morphological responses characterized as acclimation and adaptation$^{6}$. Here we show, using special sun simulators, that elevated solar UV-B doses increase the frequency of somatic homologous DNA rearrangements in Arabidopsis and tobacco plants. Increases in recombination are accompanied by a strong induction of photolyase and Rad51 gene expression. These genes are putatively involved in major DNA repair pathways, photoreactivation and recombination repair$^{7,8}$. In mutant Arabidopsis plants that are deficient in photoreactivating ultraviolet-induced cyclobutane pyrimidine dimers, recombination under elevated UV-B regimes greatly exceeds wild-type levels. Our results show that homologous recombination repair pathways might be involved in eliminating UV-B-induced DNA lesions in plants. Thus, increases in terrestrial solar UV-B radiation as forecasted for the early 21st century may affect genome stability in plants.

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Postprint §
DOI: 10.5445/IR/1000016569
Veröffentlicht am 16.09.2020
Originalveröffentlichung
DOI: 10.1038/35017595
Dimensions
Zitationen: 294
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2000
Sprache Englisch
Identifikator ISSN: 0028-0836, 1476-4687
KITopen-ID: 1000016569
Erschienen in Nature <London>
Verlag Nature Research
Band 406
Seiten 98-101
Nachgewiesen in Dimensions
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