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Species-specific double-strand break repair and genome evolution in plants

Kirik, A.; Salomon, S.; Puchta, H.

Abstract:

Even closely related eukaryotic species may differ drastically in genome size. While insertion of retroelements represents a major source of genome enlargement, the mechanism mediating species‐ specific deletions is fairly obscure. We analyzed the formation of deletions during double‐strand break (DSB) repair in Arabidopsis thaliana and tobacco, two dicotyledonous plant species differing >20‐fold in genome size. DSBs were induced by the rare cutting restriction endonuclease I‐SceI and deletions were identified by loss of function of a negative selectable marker gene containing an I‐SceI site. Whereas the partial use of micro‐homologies in junction formation was similar in both species, in tobacco 40% of the deletions were accompanied by insertions. No insertions could be detected in Arabidopsis, where larger deletions were more frequent, indicating a putative inverse correlation between genome size and the average length of deletions. Such a correlation has been postulated before by a theoretical study on the evolution of related insect genomes and our study now identifies a possible molecular cause for the phenomenon, indicating that species‐specific differences in DSB repair might indeed influence genome evolution.


Postprint §
DOI: 10.5445/IR/1000016570
Veröffentlicht am 16.09.2020
Originalveröffentlichung
DOI: 10.1093/emboj/19.20.5562
Dimensions
Zitationen: 171
Cover der Publikation
Zugehörige Institution(en) am KIT Fakultät für Chemie und Biowissenschaften – Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2000
Sprache Englisch
Identifikator ISSN: 0261-4189, 1460-2075
KITopen-ID: 1000016570
Erschienen in The EMBO journal
Verlag John Wiley and Sons
Band 19
Seiten 5562-5566
Nachgewiesen in Dimensions
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