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Towards the ideal GMP: Homologous recombination and marker gene excision

Puchta, H.

A mayor aim of biotechnology is the establishment of techniques for the precise manipulation of plant genomes. Two major efforts have been undertaken over the last dozen years, one to set up techniques for site-specific alteration of the plant genome via homologous recombination («gene targeting») and the other for the removal of selectable marker genes from transgenic plants. Unfortunately, despite multiple promising approaches that will be shortly described in this review no feasible gene targeting technique has been developed till now for crop plants. In contrast, several alternative procedures have been established successfully to remove selectable markers from plant genomes. Intriguingly besides techniques relying on transposons and site-specific recombinases, recent results indicate that homologous recombination might be a valuable alternative for the excision of marker genes.

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Postprint §
DOI: 10.5445/IR/1000016584
Veröffentlicht am 16.09.2020
DOI: 10.1078/0176-1617-01027
Zitationen: 27
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2003
Sprache Englisch
Identifikator ISSN: 0176-1617, 1618-1328
KITopen-ID: 1000016584
Erschienen in Journal of plant physiology
Verlag Elsevier
Band 160
Seiten 743-754
Nachgewiesen in Dimensions
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