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DOI: 10.1007/4243_2011_21

Fluorescent Proteins: Nature's Colourful Gifts for Live Cell Imaging

Wiedenmann, J.; D'Angelo, C.; Nienhaus, G. U.

Fluorescence of marine organisms has fascinated researchers since the early twentieth century. The successful application of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria in 1994 as genetically encoded marker resulted in a massive increase in interest for naturally fluorescent proteins. Methods are now established that allow the fast isolation of new genes encoding GFP-like proteins from marine creatures, resulting in an impressive array of glowing proteins with different biochemical and optical properties. Protein engineering has been applied to render natural variants into advanced optical tools for live cell imaging, promoting studies of protein localization and movement, gene activity, sensing of intra- and extracellular condition, and tracking of whole cells and organisms. Finally, photoactivatable proteins were discovered that enable pulse-chase experiments and live cell imaging of proteins with a resolution beyond the diffraction barrier of optical microscopy. Phylogenetic sequence analyses revealed interesting details about the molecular evolution of these proteins including the convergent evolutio ... mehr

Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Publikationstyp Buchaufsatz
Jahr 2011
Sprache Englisch
Identifikator ISBN: 978-3-642-23376-0
ISSN: 1865-1313
KITopen ID: 1000027121
Erschienen in Fluorescent Proteins II: Application of Fluorescent Protein Technolog. Ed.: G. Jung
Verlag Springer, Heidelberg
Seiten 3 - 33
Serie Springer Series on Fluorescence ; 12
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