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Differential Uptake of Functionalized Polystyrene Nanoparticles by Human Macrophages and a Monoytic Cell Line

Lunov, Oleg; Syrovets, Tatiana; Loos, Cornelia; Beil, Johanna; Delecher, Michael; Tron, Kyrylo; Nienhaus, G. Ulrich; Musyanovych, Anna; Mailaender, Volker; Landfester, Katharina; Simmet, Thomas

Tumor cell lines are often used as models for the study of nanoparticle-cell interactions. Here we demonstrate that carboxy (PS-COOH) and amino functionalized (PS-NH2) polystyrene nanoparticles of 100 nm in diameter are internalized by human macrophages, by undifferentiated and by PMA-differentiated monocytic THP-1 cells via diverse mechanisms. The uptake mechanisms also differed for all cell types and particles when analyzed either in buffer or in medium containing human serum. Macrophages internalized 4 times more PS-COOH than THP-1 cells, when analyzed in serum-containing medium. By contrast, in either medium, THP-1 cells internalized PS-NH2 more rapidly than macrophages. Using pharmacological and antisense in vitro knockdown approaches, we showed that, in the presence of serum, the specific interaction between the CD64 receptor and the particles determines the macrophage uptake of particles by phagocytosis, whereas particle internalization in THP-1 cells occurred via dynamin II-dependent endocytosis. PMA-differentiated THP-1 cells differed in their uptake mechanism from macrophages and undifferentiated THP-1 cells by internalizi ... mehr

Zugehörige Institution(en) am KIT Center for Functional Nanostructures (CFN)
Institut für Angewandte Physik (APH)
Publikationstyp Zeitschriftenaufsatz
Jahr 2011
Sprache Englisch
Identifikator ISSN: 1936-0851, 1936-086X
KITopen ID: 1000027126
Erschienen in ACS nano
Band 5
Heft 3
Seiten 1657 - 1669
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