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From EosFP to mIrisFP: Structure-based Development of Advanced Photoactivatable Marker Proteins of the GFP-family

Wiedenmann, J.; Gayda, S.; Adam, V.; Oswald, F.; Nienhaus, K.; Bourgeois, D.; Nienhaus, G. U.

Abstract:
Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non-bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse-chase experiments and super-resolution imaging. In this review, we discuss the development of advanced variants, using a structure-function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by -400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live-cell imaging.


Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Publikationstyp Zeitschriftenaufsatz
Jahr 2011
Sprache Englisch
Identifikator ISSN: 1864-063X
KITopen ID: 1000027132
Erschienen in Journal of Biophotonics
Band 4
Heft 6
Seiten 377 - 390
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