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Originalveröffentlichung
DOI: 10.1021/bm901348z
Scopus
Zitationen: 82
Web of Science
Zitationen: 77

Specific Effects of Surface Amines on Polystyrene Nanoparticles in their Interactions with Mesenchymal Stem Cells

Jiang, X.; Dausend, J.; Hafner, M.; Musyanovych, A.; Röcker, C.; Landfester, K.; Mailänder, V.; Nienhaus, G. U.

Abstract:
We have investigated the uptake of cationic polystyrene nanoparticles by mesenchymal stem cells (MSCs) using confocal fluorescence microscopy and flow cytometry. Two types of nanoparticles of about 100 nm diameter with similar zeta potentials were employed in this study, plain polystyrene (PS) nanoparticles and amino-functionalized polystyrene (NPS) nanoparticles, each carrying about 6000 amino groups on the surface. To assess the relative importance of specific endocytosis mechanisms, uptake was observed in the presence of the drugs dynasore and chlorpromazine. NPS nanoparticles were rapidly internalized and accumulated to a much higher level in MSCs than PS nanoparticles, predominantly via the main clathrin-mediated pathway. PS nanoparticles were internalized mainly via clathrin-independent endocytosis. The pronounced difference in the internalization behavior of PS and NPS nanoparticles points to specific interactions of the amino groups on the nanoparticle surface with the endocytosis machinery of the cells.


Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Publikationstyp Zeitschriftenaufsatz
Jahr 2010
Sprache Englisch
Identifikator ISSN: 1525-7797, 1526-4602
KITopen-ID: 1000027189
Erschienen in Biomacromolecules
Band 11
Heft 3
Seiten 748 - 753
Nachgewiesen in Web of Science
Scopus
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