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A Photoactivatable Marker Protein for Pulse-chase Imaging with Superresolution

Fuchs, J.; Böhme, S.; Oswald, F.; Hedde, P. N.; Krause, M.; Wiedenmann, J.; Nienhaus, G. U.

IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM).

DOI: 10.1038/nmeth.1477
Zitationen: 81
Web of Science
Zitationen: 74
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Publikationstyp Zeitschriftenaufsatz
Jahr 2010
Sprache Englisch
Identifikator ISSN: 1548-7091
KITopen-ID: 1000027192
Erschienen in Nature Methods
Band 7
Heft 8
Seiten 627 - 630
Nachgewiesen in Scopus
Web of Science
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