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Endo- and Exocytosis of Zwitterionic Quantum Dot Nanoparticles by Living Cells

Jiang, X.; Röcker, C.; Hafner, M.; Nienhaus, G. U.

Abstract:
Uptake and intracellular transport of D-penicillamine coated quantum dots (DPA-QDs) of 4 nm radius by live HeLa cells have been investigated systematically by spinning disk and 4Pi confocal microscopies. Unlike larger nanoparticles, these small DPA-QDs were observed to accumulate at the plasma membrane prior to internalization, and the uptake efficiency scaled nonlinearly with the nanoparticle concentration. Both observations indicate that a critical threshold density has to be exceeded for triggering the internalization process. By using specific inhibitors, we showed that DPA-QDs were predominantly internalized by clathrin-mediated endocytosis and to a smaller extent by macropinocytosis. Clusters of DPA-QDs were found in endosomes, which were actively transported along microtubules toward the perinuclear region. Later on, a significant fraction of endocytosed DPA-QDs were found in lysosomes, while others were actively transported to the cell periphery and exocytosed with a half-life of 21 min.



Originalveröffentlichung
DOI: 10.1021/nn101277w
Scopus
Zitationen: 192
Web of Science
Zitationen: 182
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Publikationstyp Zeitschriftenaufsatz
Jahr 2010
Sprache Englisch
Identifikator ISSN: 1936-0851
KITopen-ID: 1000027203
Erschienen in ACS Nano
Band 4
Heft 11
Seiten 6787 - 6797
Nachgewiesen in Web of Science
Scopus
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