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Verlagsausgabe
DOI: 10.5445/IR/1000027665
Veröffentlicht am 11.04.2018
Originalveröffentlichung
DOI: 10.1093/nar/gkq722
Scopus
Zitationen: 17
Web of Science
Zitationen: 16

BRCC36A is epistatic to BRCA1 in DNA crosslink repair and homologous recombination in Arabidopsis thaliana

Block-Schmidt, A.; Dukowic-Schulze, S.; Wanieck, K.; Reidt, W.; Puchta, H.

Abstract:
BRCA1 is a well-known tumor suppressor protein in mammals, involved in multiple cellular processes such as DNA repair, chromosome segregation and chromatin remodeling. Interestingly, homologs of BRCA1 and several of its complex partners are also found in plants. As the respective mutants are viable, in contrast to mammalian mutants, detailed analyses of their biological role is possible. Here we demonstrate that the model plant Arabidopsis thaliana harbors two homologs of the mammalian BRCA1 interaction partner BRCC36, AtBRCC36A and AtBRCC36B. Mutants of both genes as well as the double mutants are fully fertile and show no defects in development. We were able to show that mutation of one of the homologs, AtBRCC36A, leads to a severe defect in intra- and interchromosomal homologous recombination (HR). A HR defect is also apparent in Atbrca1 mutants. As the Atbrcc36a/Atbrca1 double mutant behaves like the single mutants of AtBRCA1 and AtBRCC36A both proteins seem to be involved in a common pathway in the regulation of HR. AtBRCC36 is also epistatic to AtBRCA1 in DNA crosslink repair. Upon genotoxic stress, AtBRCC36A is transferred in ... mehr


Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Jahr 2011
Sprache Englisch
Identifikator ISSN: 0301-5610, 0305-1048, 1362-4962
URN: urn:nbn:de:swb:90-276658
KITopen ID: 1000027665
Erschienen in Nucleic acids research
Band 39
Heft 1
Seiten 146-154
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