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URN: urn:nbn:de:swb:90-284905

Multiple trap optical tweezers for cell force measurements

Schwingel, Melanie

Abstract:
Adherent cells establish transient adhesion sites that serve as stable anchors of the cell cytoskeleton to the substrate and concomitantly allow for force transduction and cell motility. We established a multiple trap optical tweezers system for non-invasive cell force measurements at individual adhesion sites. A force study was conducted to analyze the impact of bead functionalization, adhesion site area, location, spacing, and orientation as well as the role of vinculin on adhesion formation.


Zugehörige Institution(en) am KIT Zoologisches Institut (ZOO)
Publikationstyp Hochschulschrift
Jahr 2012
Sprache Englisch
Identifikator KITopen ID: 1000028490
Verlag Karlsruhe
Abschlussart Dissertation
Fakultät Fakultät für Chemie und Biowissenschaften (CHEM-BIO)
Institut Zoologisches Institut (ZOO)
Prüfungsdaten 20.04.2012
Referent/Betreuer Prof. M. Bastmeyer
Schlagworte optical tweezers, adhesion forces, vinculin, retrograde actin flow
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