Investigation of a Folding Intermediate of RNase H Using Single Molecule FRET Spectroscopy
Abstract:
FRET studies were performed on freely diffusing RNase H molecules at pH 7.2 and ph 3.0. The application of ALEX and PIE illumination and detection schemes allowed the accumulation of donor-only free smFRET data with excellent statistics. By imposing physically sensible global restrictions on the fit of the data, an intermediate folding state can be observed and characterized.
| Zugehörige Institution(en) am KIT | Institut für Angewandte Physik (APH) |
| Publikationstyp | Hochschulschrift |
| Publikationsjahr | 2012 |
| Sprache | Englisch |
| Identifikator | urn:nbn:de:swb:90-307636 KITopen-ID: 1000030763 |
| Verlag | Karlsruher Institut für Technologie (KIT) |
| Art der Arbeit | Dissertation |
| Fakultät | Fakultät für Physik (PHYSIK) |
| Institut | Institut für Angewandte Physik (APH) |
| Prüfungsdaten | 02.11.2012 |
| Referent/Betreuer | Nienhaus, G. U. |