Mikrobielle und enzymatische Hydrolyse von cyclischen Dipeptiden (Diketopiperazine) sowie Etablierung von Analysemethoden zum Nachweis der Edukte und Produkte

The enzymatic and microbial cleavage of diketopiperazines (cyclic dipeptides) was studied. An HPLC analysis was established for quantitation of the reaction. Five approaches to identify biocatalysts for DKP degradation were investigated: peptidases, screening, strains reported for DKP hydrolysis, strains hydrolyzing other cyclic amides, and identification of novel isolates for the enantioselective DKP hydrolysis. Altogether, five bacterial strains were newly identified for DKP degradation.