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One‐Pot Synthesis of Near‐Infrared Fluorescent Gold Clusters for Cellular Fluorescence Lifetime Imaging

Shang, Li; Azadfar, Naghmeh; Stockmar, Florian; Send, Winfried; Trouillet, Vanessa; Bruns, Michael; Gerthsen, Dagmar; Nienhau, G. Ulrich

A facile strategy to synthesize water-soluble fluorescent gold nanoclusters (Au NCs) stabilized with the bidentate ligand dihydrolipoic acid (DHLA) is reported. The DHLA-capped Au NCs are characterized by UV-vis absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy. The Au NCs possess many attractive features including ultrasmall size, bright near-infrared luminescence, high colloidal stability, and good biocompatibility, making them promising imaging agents for biomedical and cellular imaging applications. Moreover, their long fluorescence lifetime (>100 ns) makes them attractive as labels in fluorescence lifetime imaging (FLIM) applications. As an example, the internalization of Au NCs by live HeLa cells is visualized using the FLIM technique.

DOI: 10.1002/smll.201100746
Zitationen: 233
Web of Science
Zitationen: 225
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Laboratorium für Elektronenmikroskopie (LEM)
Institut für Angewandte Materialien - Werkstoffprozesstechnik (IAM-WPT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2011
Sprache Englisch
Identifikator ISSN: 1613-6810, 1613-6829
KITopen-ID: 1000035845
HGF-Programm 47.03.02 (POF II, LK 01)
Erschienen in Small
Band 7
Heft 18
Seiten 2614-2620
Nachgewiesen in Web of Science
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