Untersuchung der biologischen Aktivität von Metalloxid-Partikeln in Säugerzellen mittels Hochdurchsatzmethoden

An automated high-throughput microscopy method was developed to quantify cell counts as well as cell death after exposure of different cell lines to differentially coated iron oxide nanoparticles and silica particles of various sizes. The data was then compared to those obtained by conventional toxicity assays. Spectrometry was used to determine the cellular particle dose. Uptake and localization of silica particles in cells was examined by fluorescence and transmission electron microscopy.