Novel approaches in chromatographic purification process development for low concentrated proteins in complex mixtures
Abstract:
Analytical separations, based on pH, salt gradient ion exchange chromatography, were laid out and combined with modern protein analytical technologies for the characterization of protein mixtures. Methods were used to obtain parameters necessary for purification process development / simulation. The applicability of the obtained parameters was further experimentally proven. Additionally, a method for high resolution separation and identification of protein PEGylation isoforms is demonstrated.
| Zugehörige Institution(en) am KIT | Institut für Bio- und Lebensmitteltechnik (BLT) |
| Publikationstyp | Hochschulschrift |
| Jahr | 2013 |
| Sprache | Englisch |
| Identifikator | KITopen ID: 1000037702 |
| Verlag | Karlsruhe |
| Abschlussart | Dissertation |
| Fakultät | Fakultät für Chemieingenieurwesen und Verfahrenstechnik (CIW) |
| Institut | Institut für Bio- und Lebensmitteltechnik (BLT) |
| Prüfungsdaten | 20.09.2013 |
| Referent/Betreuer | Prof. J. Hubbuch |
| Schlagworte | protein, chromatography, purification, protein analysis, process development |