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URN: urn:nbn:de:swb:90-377023

Novel approaches in chromatographic purification process development for low concentrated proteins in complex mixtures

Kröner, Frieder

Abstract:
Analytical separations, based on pH, salt gradient ion exchange chromatography, were laid out and combined with modern protein analytical technologies for the characterization of protein mixtures. Methods were used to obtain parameters necessary for purification process development / simulation. The applicability of the obtained parameters was further experimentally proven. Additionally, a method for high resolution separation and identification of protein PEGylation isoforms is demonstrated.


Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Hochschulschrift
Jahr 2013
Sprache Englisch
Identifikator KITopen ID: 1000037702
Verlag Karlsruhe
Abschlussart Dissertation
Fakultät Fakultät für Chemieingenieurwesen und Verfahrenstechnik (CIW)
Institut Institut für Bio- und Lebensmitteltechnik (BLT)
Prüfungsdaten 20.09.2013
Referent/Betreuer Prof. J. Hubbuch
Schlagworte protein, chromatography, purification, protein analysis, process development
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