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Proteome Mapping of Adult Zebrafish Marrow Neutrophils Reveals Partial Cross Species Conservation to Human Peripheral Neutrophils

Singh, Sachin Kumar 1; Sethi, Sachin 1; Aravamudhan, Sriram; Krüger, Marcus; Grabher, Clemens 1
1 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)

Abstract:

Neutrophil granulocytes are pivotal cells within the first line of host defense of the innate immune system. In this study, we have used a gel-based LC-MS/MS approach to explore the proteome of primary marrow neutrophils from adult zebrafish. The identified proteins originated from all major cellular compartments. Gene ontology analysis revealed significant association of proteins with different immune-related network and pathway maps. 75% of proteins identified in neutrophils were identified in neutrophils only when compared to neutrophil-free brain tissue. Moreover, cross-species comparison with human peripheral blood neutrophils showed partial conservation of immune-related proteins between human and zebrafish. This study provides the first zebrafish neutrophil proteome and may serve as a valuable resource for an understanding of neutrophil biology and innate immunity.


Volltext §
DOI: 10.5445/IR/1000039924
Originalveröffentlichung
DOI: 10.1371/journal.pone.0073998
Scopus
Zitationen: 8
Web of Science
Zitationen: 7
Dimensions
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2013
Sprache Englisch
Identifikator ISSN: 1932-6203
urn:nbn:de:swb:90-399243
KITopen-ID: 1000039924
HGF-Programm 47.01.01 (POF II, LK 01) Biologische Schlüselmoleküle ITG
Erschienen in PLoS ONE
Verlag Public Library of Science (PLoS)
Band 8
Heft 9
Seiten e73998/1-9
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Web of Science
Scopus
Dimensions
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