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URN: urn:nbn:de:swb:90-440783
Originalveröffentlichung
DOI: 10.1371/journal.pone.0109794
Scopus
Zitationen: 5

Temperature Effects on Bacterial Phytochrome

Njimona, Ibrahim; Yang, Rui; Lamparter, Tilman

Abstract:
Bacteriophytochromes (BphPs) are light-sensing regulatory proteins encoded in photosynthetic and non-photosynthetic bacteria. This protein class incorporate bilin as their chromophore, with majority of them bearing a light- regulated His kinase or His kinase related module in the C-terminal. We studied the His kinase actives in the temperature range of 5°C to 40°C on two BphPs, Agp1 from Agrobacterium tumefaciens and Cph1 from cyanobacterium Synechocystis PCC 6803. As reported, the phosphorylation activities of the far red (FR) irradiated form of the holoprotein is stronger than that of the red (R) irradiated form in both phytochromes. We observed for the apoprotein and FR irradiated holoprotein of Agp1 an increase in the phosphorylation activities from 5°C to 25°C and a decrease from 25°C to 40°C. At 5°C the activities of the apoprotein were significantly lower than those of the FR irradiated holoprotein, which was opposite at 40°C. A similar temperature pattern was observed for Cph1, but the maximum of the apoprotein was at 20°C while the maximum of the FR irradiated holoprotein was at 10°C. At 40°C, prolonged R irradiation leads ... mehr


Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Jahr 2014
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen ID: 1000044078
Erschienen in PLoS ONE
Band 9
Heft 10
Seiten e109794
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
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