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DOI: 10.5445/IR/1000045938
Veröffentlicht am 13.03.2018
DOI: 10.1186/1475-2859-11-64
Zitationen: 31
Web of Science
Zitationen: 30

Formation of Se (0) Nanoparticles by Duganella sp. and Agrobacterium sp. isolated from Se-laden soil of North-East Punjab, India

Bajaj, Mini; Schmidt, Susan; Winter, Josef

Background: Selenium (Se) is an essential trace element, but is toxic at high concentrations. Depending upon the geological background, the land use or on anthropogenic pollution, different amounts of Se may be present in soil. Its toxicity is related to the oxyanions selenate and selenite as they are water soluble and bioavailable. Microorganisms play an important role in Se transformations in soil and its cycling in the environment by transforming water-soluble oxyanions into water insoluble, non-toxic elemental Se (0). For this study, soil samples were collected from selenium-contaminated agricultural soils of Punjab/India to enrich and isolate microbes that interacted with the Se cycle.
Results: A mixed microbial culture enriched from the arable soil of Punjab could reduce 230 mg/l of water soluble selenite to spherical Se (0) nanoparticles during aerobic growth as confirmed by SEM-EDX. Four pure cultures (C 1, C 4, C 6, C 7) of Gram negative, oxidase and catalase positive, aerobic bacteria were isolated from this mixed microbial consortium and identified by 16 S rDNA gene sequence alignment as two strains of Duganella sp. (C 1 ... mehr

Zugehörige Institution(en) am KIT Institut für Ingenieurbiologie und Biotechnologie des Abwassers (IBA)
Publikationstyp Zeitschriftenaufsatz
Jahr 2012
Sprache Englisch
Identifikator ISSN: 1475-2859
URN: urn:nbn:de:swb:90-459388
KITopen ID: 1000045938
Erschienen in Microbial cell factories
Band 11
Seiten Article Nr. 64
Schlagworte Bacteria isolation, 16 S rDNA alignment, Duganella sp., Agrobacterium sp., Selenite reduction, SEM-EDX analysis, Biosynthesis, Se(0) nanoparticles
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