KIT | KIT-Bibliothek | Impressum

Characterization of newly isolated oleaginous yeasts - Cryptococcus podzolicus, Trichosporon porosum and pichia segobiensis

Schulze, I.; Hansen, S.; Großhans, S.; Rudszuck, T.; Ochsenreither, K.; Syldatk, C.; Neumann, A.

Abstract:
The yeast strains Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis were isolated from soil samples and identified as oleaginous yeast strains beneficial for the establishment of microbial production processes for sustainable lipid production suitable for several industrial applications. When cultured in bioreactors with glucose as the sole carbon source C. podzolicus yielded 31.8% lipid per dry biomass at 20°C, while T. porosum yielded 34.1% at 25°C and P. segobiensis 24.6% at 25°C. These amounts correspond to lipid concentrations of 17.97 g/L, 17.02 g/L and 12.7 g/L and volumetric productivities of 0.09 g/Lh, 0.1 g/Lh and 0.07 g/Lh, respectively. During the culture of C. podzolicus 30 g/l gluconic acid was detected as by-product in the culture broth and 12 g/L gluconic acid in T. porosum culture. The production of gluconic acid was eliminated for both strains when glucose was substituted by xylose as the carbon source. Using xylose lipid yields were 11.1 g/L and 13.9 g/L, corresponding to 26.8% and 33.4% lipid per dry biomass and a volumetric productivity of 0.07 g/Lh and 0.09 g/Lh, for C. podzolicus and T. poro ... mehr


Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
House of Competence (HoC)
Publikationstyp Zeitschriftenaufsatz
Jahr 2014
Sprache Englisch
Identifikator DOI: 10.1186/s13568-014-0024-0
ISSN: 2191-0855
URN: urn:nbn:de:swb:90-463905
KITopen ID: 1000046390
Erschienen in AMB Express
Band 4
Heft 24
Seiten 1-10
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte Screening; Oleaginous yeasts; Microbial lipid production; Cryptococcus podzolicus; Trichosporon porosum; Pichia segobiensis
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft KITopen Landing Page