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Cytotoxicity and genotoxicity of nano - and microparticulate copper oxide: Role of solubility and intracellular bioavailability

Semisch, A.; Ohle, J.; Witt, B.; Hartwig, A.


Nano- or microscale copper oxide particles (CuO NP, CuO MP) are increasingly applied as catalysts or antimicrobial additives. This increases the risk of adverse health effects, since copper ions are cytotoxic under overload conditions.


The extra- and intracellular bioavailability of CuO NP and CuO MP were explored. In addition, different endpoints related to cytotoxicity as well as direct and indirect genotoxicity of the copper oxides and copper chloride (CuCl2) were compared.


Comprehensively characterized CuO NP and CuO MP were analysed regarding their copper ion release in model fluids. In all media investigated, CuO NP released far more copper ions than CuO MP, with most pronounced dissolution in artificial lysosomal fluid. CuO NP and CuCl2 caused a pronounced and dose dependent decrease of colony forming ability (CFA) in A549 and HeLa S3 cells, whereas CuO MP exerted no cytotoxicity at concentrations up to 50 mu g/mL. Cell death induced by CuO NP was at least in part due to apoptosis, as determined by subdiploid DNA as well as via translocation of the apoptosis inducing factor (AIF) into the cell nucleus. ... mehr

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DOI: 10.5445/IR/1000046391
DOI: 10.1186/1743-8977-11-10
Zitationen: 70
Web of Science
Zitationen: 69
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Jahr 2014
Sprache Englisch
Identifikator ISSN: 1743-8977
KITopen-ID: 1000046391
Erschienen in Particle and Fibre Toxicology
Band 11
Heft 1
Seiten 10
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte Copper oxide nanoparticles; Copper oxide microparticles; Copper chloride; Bioavailability; Intracellular distribution; Cytotoxicity; Genotoxicity; Poly(ADP-ribosyl)ation; Apoptosis
Nachgewiesen in Web of Science
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