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Screening and monitoring microbial xenobiotics' biodegradation by rapid, inexpensive and easy to perform microplate UV-absorbance measurements

Herzog, B.; Lemmer, H.; Horn, H.; Müller, E.


Evaluation of xenobiotics biodegradation potential, shown here for benzotriazoles (corrosion inhibitors) and sulfamethoxazole (sulfonamide antibiotic) by microbial communities and/or pure cultures normally requires time intensive and money consuming LC/GC methods that are, in case of laboratory setups, not always needed.


The usage of high concentrations to apply a high selective pressure on the microbial communities/pure cultures in laboratory setups, a simple UV-absorbance measurement (UV-AM) was developed and validated for screening a large number of setups, requiring almost no preparation and significantly less time and money compared to LC/GC methods. This rapid and easy to use method was evaluated by comparing its measured values to LC-UV and GC-MS/MS results. Furthermore, its application for monitoring and screening unknown activated sludge communities (ASC) and mixed pure cultures has been tested and approved to detect biodegradation of benzotriazole (BTri), 4- and 5-tolyltriazole (4-TTri, 5-TTri) as well as SMX.


In laboratory setups, xenobiotics concentrations above 1.0 mg L-1 wi ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000046395
Veröffentlicht am 12.11.2018
DOI: 10.1186/1756-0500-7-101
Zitationen: 4
Zugehörige Institution(en) am KIT Engler-Bunte-Institut (EBI)
Publikationstyp Zeitschriftenaufsatz
Jahr 2014
Sprache Englisch
Identifikator ISSN: 1756-0500
KITopen-ID: 1000046395
Erschienen in BMC Research Notes
Band 7
Heft 1
Seiten 101
Schlagworte Xenobiotics; Biodegradation; Microplate measurement; UV-absorbance; Benzotriazoles; Sulfamethoxazole
Nachgewiesen in Scopus
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