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Interaction of Cm(iii) and Am(iii) with human serum transferrin studied by time-resolved laser fluorescence and EXAFS spectroscopy

Bauer, Nicole; Fröhlich, Daniel R.; Panak, Petra J.

Abstract:
The complexation of Cm(iii) with human serum transferrin was investigated in a pH range from 3.5 to 11.0 using time-resolved laser fluorescence spectroscopy (TRLFS). At pH [greater-than-or-equal] 7.4 Cm(iii) is incorporated at the Fe(iii) binding site of transferrin whereas at lower pH a partially bound Cm(iii) transferrin species is formed. At physiological temperature (310 K) at pH 7.4{,} about 70% of the partially bound and 30% of the incorporated Cm(iii) transferrin species are present in solution. The Cm(iii) results obtained by TRLFS are in very good agreement with Am(iii) EXAFS results{,} confirming the incorporation of Am(iii) at the Fe(iii) binding site at pH 8.5.

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Volltext §
DOI: 10.5445/IR/1000046417
Originalveröffentlichung
DOI: 10.1039/C3DT53371A
Scopus
Zitationen: 15
Web of Science
Zitationen: 15
Zugehörige Institution(en) am KIT Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Institut für Nukleare Entsorgung (INE)
Publikationstyp Zeitschriftenaufsatz
Jahr 2014
Sprache Englisch
Identifikator ISSN: 0300-9246, 1364-5447, 1470-479X, 1472-7773, 1477-9226, 1477-9234
urn:nbn:de:swb:90-464171
KITopen-ID: 1000046417
HGF-Programm 32.27.04 (POF II, LK 01)
Erschienen in Dalton transactions
Band 43
Heft 18
Seiten 6689-6700
Bemerkung zur Veröffentlichung RSC geförderter Open Access-Artikel
Nachgewiesen in Web of Science
Scopus
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