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DOI: 10.5445/IR/1000046417

Interaction of Cm(iii) and Am(iii) with human serum transferrin studied by time-resolved laser fluorescence and EXAFS spectroscopy

Bauer, Nicole; Fröhlich, Daniel R.; Panak, Petra J.

Abstract:
The complexation of Cm(iii) with human serum transferrin was investigated in a pH range from 3.5 to 11.0 using time-resolved laser fluorescence spectroscopy (TRLFS). At pH [greater-than-or-equal] 7.4 Cm(iii) is incorporated at the Fe(iii) binding site of transferrin whereas at lower pH a partially bound Cm(iii) transferrin species is formed. At physiological temperature (310 K) at pH 7.4{,} about 70% of the partially bound and 30% of the incorporated Cm(iii) transferrin species are present in solution. The Cm(iii) results obtained by TRLFS are in very good agreement with Am(iii) EXAFS results{,} confirming the incorporation of Am(iii) at the Fe(iii) binding site at pH 8.5.


Zugehörige Institution(en) am KIT Institut für Nukleare Entsorgung (INE)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Publikationstyp Zeitschriftenaufsatz
Jahr 2014
Sprache Englisch
Identifikator ISSN: 0300-9246, 1364-5447, 1470-479X, 1472-7773, 1477-9226, 1477-9234
URN: urn:nbn:de:swb:90-464171
KITopen ID: 1000046417
HGF-Programm 32.27.04; LK 01
Erschienen in Dalton transactions
Band 43
Heft 18
Seiten 6689-6700
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