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URN: urn:nbn:de:swb:90-469686
DOI: 10.1371/journal.pone.0117300
Zitationen: 16
Web of Science
Zitationen: 14

Multi-channel microfluidic biosensor platform applied for online monitoring and screening of biofilm formation and activity

Bruchmann, J.; Sachsenheimer, K.; Rapp, B.E.; Schwartz, T.

Bacterial colonization of surfaces and interfaces has a major impact on various areas including biotechnology, medicine, food industries, and water technologies. In most of these areas biofilm development has a strong impact on hygiene situations, product quality, and process efficacies. In consequence, biofilm manipulation and prevention is a fundamental issue to avoid adverse impacts. For such scenario online, non-destructive biofilm monitoring systems become important in many technical and industrial applications. This study reports such a system in form of a microfluidic sensor platform based on the combination of electrical impedance spectroscopy and amperometric current measurement, which allows sensitive online measurement of biofilm formation and activity. A total number of 12 parallel fluidic channels enable real-time online screening of various biofilms formed by different Pseudomonas aeruginosa and Stenotrophomonas maltophilia strains and complex mixed population biofilms. Experiments using disinfectant and antibiofilm reagents demonstrate that the biofilm sensor is able to discriminate between inactivation/killing of bac ... mehr

Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 1932-6203

KITopen-ID: 1000046968
HGF-Programm 47.02.06 (POF III, LK 01)
Erschienen in PLoS ONE
Band 10
Heft 2
Seiten e0117300
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Web of Science
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