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DOI: 10.1007/978-3-662-43899-2_10

Continuous Magnetic Extraction for Protein Purification

Paulus, Anja; Franzreb, Matthias

This study describes the design and development of an in situ magnetic separation process (ISMS) based on the use of ion exchange functionalized magnetic particles. ISMS as a tool for in situ product removal (ISPR) has the potential to increase the performance of a biotechnological production process comprehensively by improving the bioprocess itself and the downstream processing simultaneously. The successful implementation of this concept requires the systematic examination and optimization of the different ISMS subsystem. Hence, this report presents detailed characterization data for the magnetic particle system, the magnetic separator, and the bioprocess. For each system, specific requirements were defined and subsequently applied to identify suitable process components. In this context always economic considerations were also accounted to enable the application of this new process as cost-efficiently as possible. Especially the selection and modification of a magnetic particle system and the development of a low-cost magnetic separator were subjected to these basic criteria. For the final verification of the ISMS effects, all c ... mehr

Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Buchaufsatz
Jahr 2014
Sprache Englisch
Identifikator ISBN: 978-3-662-43898-5
KITopen ID: 1000047526
HGF-Programm 47.03.03; LK 01
Erschienen in Upscaling of bio-nano-processes : selective bioseparation by magnetic particles. Ed.: H. Nirschl
Verlag Springer, Heidelberg
Seiten 189-211
Serie Lecture notes in bioengineering
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