KIT | KIT-Bibliothek | Impressum | Datenschutz

Impacts of different exposure scenarios on transcript abundances in Danio rerio embryos when investigating the toxicological burden of riverine sediments

Bluhm, K.; Otte, J.C.; Yang, L.; Zinsmeister, C.; Legradi, J.; Keiter, S.; Kosmehl, T.; Braunbeck, T.; Strähle, U.; Hollert, H.

Purpose: Recently, a proof-of-concept study revealed the suitability of transcriptome analyses to obtain and assess changes in the abundance of transcripts in zebrafish (Danio rerio) embryos after exposure to organic sediment extracts. The present study investigated changes in the transcript abundance in zebrafish embryos exposed to whole sediment samples and corresponding organic extracts in order to identify the impact of different exposure pathways on sediment toxicity.
Materials and Methods: Danio rerio embryos were exposed to sublethal concentrations of three sediment samples from the Danube River, Germany. The sediment samples were investigated both as freeze-dried samples and as organic extracts. Silica dust and a process control of the extraction procedure were used as references. After exposure, mRNA was isolated and changes in profiles of gene expression levels were examined by an oligonucleotide microarray. The microarray results were compared with bioassays, chemical analysis of the sediments and profiles of gene expression levels induced by several single substances.
Results and Discussion: The microarray approach elucidated significant changes in the abundance of transcripts in exposed zebrafish embryos compared to the references. ... mehr

Open Access Logo

Verlagsausgabe §
DOI: 10.5445/IR/1000049207
Veröffentlicht am 13.04.2018
DOI: 10.1371/journal.pone.0106523
Zitationen: 8
Web of Science
Zitationen: 7
Zitationen: 10
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2014
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen-ID: 1000049207
HGF-Programm 47.03.01 (POF II, LK 01) Biofunktionale Oberflächen ITG
Erschienen in PLoS one
Verlag Public Library of Science (PLoS)
Band 9
Heft 9
Seiten e106523
Nachgewiesen in Dimensions
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page