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DOI: 10.5445/IR/1000049238
Veröffentlicht am 22.03.2018
DOI: 10.1371/journal.pone.0089512
Zitationen: 10
Web of Science
Zitationen: 9

Effect of Radiographic Contrast Media on the Spectrin/Band3-Network of the Membrane Skeleton of Erythrocytes

Franke, Ralf-Peter; Scharnweber, Tim; Fuhrmann, Rosemarie; Wenzel, Folker; Krüger, Anne; Mrowietz, Christof; Jung, Friedrich

The membrane of red blood cells consists of a phospholipid bilayer with embedded membrane proteins and is associated on the cytoplasmatic side with a network of proteins, the membrane skeleton. Band3 has an important role as centre of the functional complexes e.g. gas exchange complex and as element of attachment for the membrane skeleton maintaining membrane stability and flexibility. Up to now it is unclear if band3 is involved in the morphology change of red blood cells after contact with radiographic contrast media. The study revealed for the first time that Iopromide induced markedly more severe alterations of the membrane skeleton compared to Iodixanol whose effects were similar to erythrocytes suspended in autologous plasma. A remarkable clustering of band3 was found associated with an accumulation of band3 in spicules and also a sequestration of band3 to the extracellular space. This was evidently accompanied by a gross reduction of functional band3 complexes combined with a dissociation of spectrin from band3 leading to a loss of homogeneity of the spectrin network. It could be demonstrated for the first time that RCM not o ... mehr

Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2014
Sprache Englisch
Identifikator ISSN: 1932-6203
URN: urn:nbn:de:swb:90-492386
KITopen ID: 1000049238
HGF-Programm 47.03.02; LK 01
Erschienen in PLoS one
Band 9
Heft 2
Seiten e89512
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