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Foam-free Production of Surfactin via Anerobic Fermentation of Bacillus subtilis DSM 10T

Willenbacher, Judit; Rau, Jens-Tilman; Rogalla, Jonas; Syldatk, Christoph; Hausmann, Rudolf

Abstract:
Surfactin is one of the most popular biosurfactants due to its numerous potential applications. The usually aerobic production via fermentation of Bacillus subtilis is accompanied by vigorous foaming which leads to complex constructions and great expense. Therefore it is reasonable to search for alternative foam-free production processes. The current study introduces a novel approach to produce Surfactin in a foam-free process applying a strictly anaerobic bioreactor cultivation. The process was performed several times with different glucose concentrations in mineral salt medium. The fermentations were analyzed regarding specific (qSurfactin, vol. qSurfactin) and overall product yields (YP/X, YP/S) as well as substrate utilization (YX/S). Fermentations in which 2.5 g/L glucose were employed proofed to be the most effective, reaching product yields of YP/X = 0.278 g/g. Most interesting, the product yields exceeded classical aerobic fermentations, in which foam fractionation was applied. Additionally, values for specific production rate qSurfactin (0.005 g/(g∙h)) and product yield per consumed substrate (YP/S = 0.033 g/g) surpass resu ... mehr


Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator DOI: 10.1186/s13568-015-0107-6
ISSN: 2191-0855
URN: urn:nbn:de:swb:90-494638
KITopen ID: 1000049463
Erschienen in AMB Express
Band 5
Heft 1
Seiten 21/1-9
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte Anaerobic fermentation; Bacillus subtilis; Foam-free; Surfactin
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