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Synthetic enzyme supercomplexes: Co-immobilization of enzyme cascades

Kazenwadel, F.; Franzreb, M.; Rapp, B.E.

A sustainable alternative to traditional chemical synthesis is the use of enzymes as biocatalysts. Using enzymes, different advantages such as mild reaction conditions and high turnover rates are combined. However, the approach of using soluble enzymes suffers from the fact that enzymes have to be separated from the product post-synthesis and can be inactivated by this process. Therefore, enzymes are often immobilized to solid carriers to enable easy separation from the product as well as stabilization of the enzyme structure. In order to mimic the metabolic pathways of living cells and thus to create more complex bioproducts in a cell-free manner, a series of consecutive reactions can be realized by applying whole enzyme cascades. As enzymes from different host organisms can be combined, this offers enormous opportunities for creating advanced metabolic pathways that do not occur in nature. When immobilizing this enzyme cascades in a co-localized pattern a further advantage emerges: as the product of the previous enzyme is directly transferred to its co-immobilized subsequent catalyst, the overall performance of the cascade can be enhanced. ... mehr

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DOI: 10.5445/IR/1000049689
DOI: 10.1039/c5ay00453e
Zitationen: 31
Web of Science
Zitationen: 31
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 1759-9660
KITopen-ID: 1000049689
HGF-Programm 47.02.06 (POF III, LK 01)
Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in Analytical Methods
Band 7
Heft 10
Seiten 4030-4037
Bemerkung zur Veröffentlichung RSC geförderter Open Access-Artikel
Nachgewiesen in Web of Science
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