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Investigation of Cell Growth and Chlorophyll a Content of the Coccolithophorid Alga Emiliania huxleyi by Using Simple Bench-Top Flow Cytometry

Hariskos, Ioanna; Rubner, Tobias; Posten, Clemens

The coccolithophorid alga Emiliania huxleyi produces micro-structured calcite particles, which are called coccoliths. Due to their unique and sophisticated structure, coccoliths are highly promising for different industrial applications, such as paper manufacturing, color and lacquer preparation. The mass production of coccoliths requires the evaluation of optimum cultivation conditions. This study investigates the impact of varying irradiance (10-1500 µmol m-² s-1) on growth and chlorophyll a content of two calcifying strains CCMP371 and RCC1216 as well as on the non-calcifying strain RCC1217 (haploid form of RCC1217). The light kinetics contradicts the popular opinion, that E. huxleyi is an extraordinarily light tolerating alga in general. Photoinhibition was already observed at irradiance >500 µmol m-2 s-1 in the case of the calcifying strains. Furthermore, light requirements to grow at maximum growth rate, as well as thresholds towards photoinhibition were considerably different between calcifying and non-calcifying strains. The haplont required significantly higher irradiance to reach maximum µspec (>200 µmol m-2 s-1), while being much more tolerant to towards photoinhibition, which occurred not until 800 µmol m-2 s-1. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000049764
DOI: 10.4172/2155-9821.1000234
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 2155-9821
KITopen-ID: 1000049764
Erschienen in Journal of Bioprocessing & Biotechniques
Band 5
Seiten Article no 1000234
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