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Superresolution microscopy reveals a dynamic picture of cell polarity maintenance during directional growth

Ishitsuka, Y. 1; Savage, N.; Li, Y. 1; Bergs, A. 2; Grün, N. 2; Kohler, D. 1; Donnelly, R.; Nienhaus, G. U. ORCID iD icon 1,3,4; Fischer, R. 2; Takeshita, N. 2
1 Institut für Angewandte Physik (APH), Karlsruher Institut für Technologie (KIT)
2 Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut für Technologie (KIT)
3 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)
4 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)

Abstract:

Polar (directional) cell growth, a key cellular mechanism shared among a wide range of species, relies on targeted insertion of new material at specific locations of the plasma membrane. How these cell polarity sites are stably maintained during massive membrane insertion has remained elusive. Conventional live-cell optical microscopy fails to visualize polarity site formation in the crowded cell membrane environment because of its limited resolution. We have used advanced live-cell imaging techniques to directly observe the localization, assembly, and disassembly processes of cell polarity sites with high spatiotemporal resolution in a rapidly growing filamentous fungus, Aspergillus nidulans. We show that the membrane-associated polarity site marker TeaR is transported on microtubules along with secretory vesicles and forms a protein cluster at that point of the apical membrane where the plus end of the microtubule touches. There, a small patch of membrane is added through exocytosis, and the TeaR cluster gets quickly dispersed over the membrane. There is an incessant disassembly and reassembly of polarity sites at the growth zone, and each new polarity site locus is slightly offset from preceding ones. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000050699
Originalveröffentlichung
DOI: 10.1126/sciadv.1500947
Scopus
Zitationen: 32
Web of Science
Zitationen: 30
Dimensions
Zitationen: 35
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Institut für Angewandte Physik (APH)
Institut für Nanotechnologie (INT)
Institut für Toxikologie und Genetik (ITG)
Universität Karlsruhe (TH) – Interfakultative Einrichtungen (Interfakultative Einrichtungen)
Karlsruhe School of Optics & Photonics (KSOP)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 2375-2548
urn:nbn:de:swb:90-506998
KITopen-ID: 1000050699
HGF-Programm 43.23.01 (POF III, LK 01) Advanced Optical Lithography+Microscopy
Erschienen in Science advances
Verlag American Association for the Advancement of Science (AAAS)
Band 1
Heft 10
Seiten e1500947
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
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Web of Science
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