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URN: urn:nbn:de:swb:90-506998
DOI: 10.1126/sciadv.1500947

Superresolution microscopy reveals a dynamic picture of cell polarity maintenance during directional growth

Ishitsuka, Y.; Savage, N.; Li, Y.; Bergs, A.; Grün, N.; Kohler, D.; Donnelly, R.; Nienhaus, G.U.; Fischer, R.; Takeshita, N.

Polar (directional) cell growth, a key cellular mechanism shared among a wide range of species, relies on targeted insertion of new material at specific locations of the plasma membrane. How these cell polarity sites are stably maintained during massive membrane insertion has remained elusive. Conventional live-cell optical microscopy fails to visualize polarity site formation in the crowded cell membrane environment because of its limited resolution. We have used advanced live-cell imaging techniques to directly observe the localization, assembly, and disassembly processes of cell polarity sites with high spatiotemporal resolution in a rapidly growing filamentous fungus, Aspergillus nidulans. We show that the membrane-associated polarity site marker TeaR is transported on microtubules along with secretory vesicles and forms a protein cluster at that point of the apical membrane where the plus end of the microtubule touches. There, a small patch of membrane is added through exocytosis, and the TeaR cluster gets quickly dispersed over the membrane. There is an incessant disassembly and reassembly of polarity sites at the growth zone, ... mehr

Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Nanotechnologie (INT)
Institut für Toxikologie und Genetik (ITG)
Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 2375-2548
KITopen ID: 1000050699
HGF-Programm 43.23.01; LK 01
Erschienen in Science Advances
Band 1
Heft 10
Seiten e1500947
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