KIT | KIT-Bibliothek | Impressum | Datenschutz

Superresolution microscopy reveals a dynamic picture of cell polarity maintenance during directional growth

Ishitsuka, Y.; Savage, N.; Li, Y.; Bergs, A.; Grün, N.; Kohler, D.; Donnelly, R.; Nienhaus, G.U.; Fischer, R.; Takeshita, N.

Abstract:
Polar (directional) cell growth, a key cellular mechanism shared among a wide range of species, relies on targeted insertion of new material at specific locations of the plasma membrane. How these cell polarity sites are stably maintained during massive membrane insertion has remained elusive. Conventional live-cell optical microscopy fails to visualize polarity site formation in the crowded cell membrane environment because of its limited resolution. We have used advanced live-cell imaging techniques to directly observe the localization, assembly, and disassembly processes of cell polarity sites with high spatiotemporal resolution in a rapidly growing filamentous fungus, Aspergillus nidulans. We show that the membrane-associated polarity site marker TeaR is transported on microtubules along with secretory vesicles and forms a protein cluster at that point of the apical membrane where the plus end of the microtubule touches. There, a small patch of membrane is added through exocytosis, and the TeaR cluster gets quickly dispersed over the membrane. There is an incessant disassembly and reassembly of polarity sites at the growth zone, ... mehr

Open Access Logo


Verlagsausgabe §
DOI: 10.5445/IR/1000050699
Originalveröffentlichung
DOI: 10.1126/sciadv.1500947
Scopus
Zitationen: 13
Web of Science
Zitationen: 12
Coverbild
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Angewandte Biowissenschaften (IAB)
Institut für Toxikologie und Genetik (ITG)
Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 2375-2548
urn:nbn:de:swb:90-506998
KITopen-ID: 1000050699
HGF-Programm 43.23.01 (POF III, LK 01)
Erschienen in Science advances
Band 1
Heft 10
Seiten e1500947
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Scopus
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page