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A versatile microarray platform for capturing rare cells

Brinkmann, F.; Hirtz, M.; Haller, A.; Gorges, T.M.; Vellekoop, M.J.; Riethdorf, S.; Müller, V.; Pantel, K.; Fuchs, H.

Analyses of rare events occurring at extremely low frequencies in body fluids are still challenging. We established a versatile microarray-based platform able to capture single target cells from large background populations. As use case we chose the challenging application of detecting circulating tumor cells (CTCs) - about one cell in a billion normal blood cells. After incubation with an antibody cocktail, targeted cells are extracted on a microarray in a microfluidic chip. The accessibility of our platform allows for subsequent recovery of targets for further analysis. The microarray facilitates exclusion of false positive capture events by co-localization allowing for detection without fluorescent labelling. Analyzing blood samples from cancer patients with our platform reached and partly outreached gold standard performance, demonstrating feasibility for clinical application. Clinical researchers free choice of antibody cocktail without need for altered chip manufacturing or incubation protocol, allows virtual arbitrary targeting of capture species and therefore wide spread applications in biomedical sciences.

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DOI: 10.5445/IR/1000050795
DOI: 10.1038/srep15342
Zitationen: 23
Web of Science
Zitationen: 21
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000050795
HGF-Programm 43.22.03 (POF III, LK 01)
Printed Materials and Systems
Erschienen in Scientific reports
Band 5
Seiten 15342
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Scopus
Web of Science
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