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Verlagsausgabe
DOI: 10.5445/IR/1000051787
Veröffentlicht am 17.04.2018
Originalveröffentlichung
DOI: 10.1186/2191-0855-2-28
Scopus
Zitationen: 10
Web of Science
Zitationen: 6

Influence of pH and carbon to nitrogen ratio on mycotoxin production by Alternaria alternata in submerged cultivation

Brzonkalik, Katrin; Hümmer, Dominik; Syldatk, Christoph; Neumann, Anke

Abstract:
Production of the Alternaria mycotoxins alternariol (AOH), alternariol monomethylether (AME) and tenuazonic acid (TA) by Alternaria alternata DSM 12633 was influenced by pH and carbon to nitrogen (C:N) ratio of the growth medium both in shaking flasks and bioreactor cultivation. The impact of medium pH on mycotoxin production was studied in the range of pH 3.5 - 8. pH values above 5.5 led to a decreased mycotoxin production or inhibited mycotoxin formation completely whereas an acidic pH in the range of 4.0-4.5 was optimal for mycotoxin production. The influence of the C:N ratio was evaluated over the range of 24 to 96. Glucose was used as carbon source and its concentration was altered while nitrogen concentration was kept constant. Growth kinetics and mycotoxin production parameters were studied depending on different C:N ratios. With increasing initial glucose concentration fungal biomass did increase but the maximum specific growth rate was not influenced. The optimal initial C:N ratio for attaining highest mycotoxin concentrations was 72. A higher C:N ratio did not further enhance mycotoxin production.


Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2012
Sprache Englisch
Identifikator ISSN: 2191-0855
URN: urn:nbn:de:swb:90-517871
KITopen ID: 1000051787
Erschienen in AMB Express
Band 2
Heft 1
Seiten ArticleNr. 28
Schlagworte Alternaria alternata, Mycotoxins, pH value, C:N ratio, Submerged cultivation
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