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Uniparental mitochondrial DNA inheritance is not affected in Ustilago maydis Δatg11 mutants blocked in mitophagy

Wagner-Vogel, G. 1; Lämmer, F. 1; Kämper, J. 1; Basse, C. W. 1
1 Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut für Technologie (KIT)

Abstract:

Background: Maternal or uniparental inheritance (UPI) of mitochondria is generally observed in sexual eukaryotes, however, the underlying mechanisms are diverse and largely unknown. Recently, based on the use of mutants blocked in autophagy, it has been demonstrated that autophagy is required for strict maternal inheritance in the nematode Caenorhabditis elegans. Uniparental mitochondrial DNA (mtDNA) inheritance has been well documented for numerous fungal species, and in particular, has been shown to be genetically governed by the mating-type loci in the isogamous species Cryptococcus neoformans, Phycomyces blakesleeanus and Ustilago maydis. Previously, we have shown that the a2 mating-type locus gene lga2 is decisive for UPI during sexual development of U. maydis. In axenic culture, conditional overexpression of lga2 triggers efficient loss of mtDNA as well as mitophagy. To assess a functional relationship, we have investigated UPI in U. maydis Δatg11 mutants, which are blocked in mitophagy. Results: This study has revealed that Äatg11 mutants are not affected in pathogenic development and this has allowed us to analyse UPI under comparable developmental conditions between mating-compatible wild-type and mutant strain combinations. ... mehr


Volltext §
DOI: 10.5445/IR/1000052016
Originalveröffentlichung
DOI: 10.1186/s12866-015-0358-z
Scopus
Zitationen: 8
Web of Science
Zitationen: 8
Dimensions
Zitationen: 11
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 1471-2180
urn:nbn:de:swb:90-520164
KITopen-ID: 1000052016
Erschienen in BMC Microbiology
Verlag Springer Fachmedien Wiesbaden
Band 15
Heft 1
Seiten 23
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagwörter Mitophagy, Uniparental mitochondrial inheritance, mtDNA, Ustilago maydis, Fungi, Atg11
Nachgewiesen in Dimensions
Scopus
Web of Science
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