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DOI: 10.5445/IR/1000052538
Originalveröffentlichung
DOI: 10.1038/srep18006
Scopus
Zitationen: 15
Web of Science
Zitationen: 17

Monomeric Garnet, a far-red fluorescent protein for live-cell STED imaging

Hense, A.; Prunsche, B.; Gao, P.; Ishitsuka, Y.; Nienhaus, K.; Nienhaus, G.U.

Abstract:
The advancement of far-red emitting variants of the green fluorescent protein (GFP) is crucially important for imaging live cells, tissues and organisms. Despite notable efforts, far-red marker proteins still need further optimization to match the performance of their green counterparts. Here we present mGarnet, a robust monomeric marker protein with far-red fluorescence peaking at 670 nm. Thanks to its large extinction coefficient of 95,000 M-1 cm-1, mGarnet can be efficiently excited with 640-nm light on the red edge of its 598-nm excitation band. A large Stokes shift allows essentially the entire fluorescence emission to be collected even with 640-nm excitation, counterbalancing the lower fluorescence quantum yield of mGarnet, 9.1%, that is typical of far-red FPs. We demonstrate an excellent performance as a live-cell fusion marker in STED microscopy, using 640 nm excitation and 780 nm depletion wavelengths.


Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Nanotechnologie (INT)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 2045-2322
URN: urn:nbn:de:swb:90-525383
KITopen-ID: 1000052538
HGF-Programm 43.23.01 (POF III, LK 01)
Erschienen in Scientific Reports
Band 5
Seiten 18006/1-10
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Scopus
Web of Science
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