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Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses

Silve, A.; Leray, I.; Poignard, C.; Mir, L.M.

Abstract:
The impact of external medium conductivity on the efficiency of the reversible permeabilisation caused by pulsed electric fields was investigated. Pulses of 12 ns, 102 ns or 100 Î 1/4s were investigated. Whenever permeabilisation could be detected after the delivery of one single pulse, media of lower conductivity induced more efficient reversible permeabilisation and thus independently of the medium composition. Effect of medium conductivity can however be hidden by some saturation effects, for example when pulses are cumulated (use of trains of 8 pulses) or when the detection method is not sensitive enough. This explains the contradicting results that can be found in the literature. The new data are complementary to those of one of our previous study in which an opposite effect of the conductivity was highlighted. It stresses that the conductivity of the medium influences the reversible permeabilization by several ways. Moreover, these results clearly indicate that electropermeabilisation does not linearly depend on the energy delivered to the cells.


Zugehörige Institution(en) am KIT Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator DOI: 10.1038/srep19957
ISSN: 2045-2322
URN: urn:nbn:de:swb:90-525407
KITopen ID: 1000052540
HGF-Programm 35.98.30; LK 01
Erschienen in Scientific Reports
Band 6
Seiten 19957
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
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