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Solid-material-based Coupling Efficiency Analyzed with Time-of-Flight Secondary Ion Mass Spectrometry

Muenster, Bastian; Welle, Alexander; Ridder, Barbara; Althuon, Daniela; Striffler, Jakob; Foertsch, Tobias C.; Hahn, Lothar; Thelen, Richard; Stadler, Volker; Nesterov-Mueller, Alexander; Breitling, Frank; Loeffler, Felix F.

Abstract (englisch):
The coupling behavior of a microparticle embedded amino acid active-ester into a Poly(ethylene glycol)methacrylate-film, synthesized onto a silicon wafer by a grafting from approach, is characterized using dynamic time-of-flight secondary ion mass spectrometry (ToF-SIMS) to analyze the 3d distribution of the amino acids in the polymer film. Besides standard solid phase peptide synthesis, employing solubilized amino acids in a solvent, we used solid polymer microparticles, incorporating the amino acids. These microparticles were especially designed for a new technique to produce high-density combinatorial peptide microarrays: upon heating, the particles become viscous, which releases the embedded amino acids to diffuse and couple to the surface. In the scope of the development of this new particle-based application, ToF-SIMS is used to analyze a complex chemically modified polymer surface layer. Due to depth profile measurements, it is possible to investigate the particle-based coupling reaction not only on the surface, but also into the depth of the PEGMA film.

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Volltext §
DOI: 10.5445/IR/1000052677
DOI: 10.1016/j.apsusc.2015.10.223
Zitationen: 3
Web of Science
Zitationen: 3
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Institut für Funktionelle Grenzflächen (IFG)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator ISSN: 0169-4332, 1873-5584
KITopen-ID: 1000052677
HGF-Programm 47.01.03 (POF III, LK 01)
Erschienen in Applied surface science
Band 360
Seiten 306–314
Nachgewiesen in Scopus
Web of Science
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