Microstructure-Assisted Particle Pattern Generation for High Density Peptide Arrays

We developed particle patterning processes on microstructured surfaces for the combinatorial synthesis of high density peptide arrays. We worked with polymer microparticles with embedded amino acids derivatives and solid substrates with cylindrical microcavities. Using laser-based method, we demonstrated combinatorial particle patterning with 1 million spots/cm². With the so-called stochastic approach, we synthesized dipeptide array with a density of 25 million peptide spots/cm².