KIT | KIT-Bibliothek | Impressum | Datenschutz

Active multi-point microrheology of cytoskeletal networks

Paust, Tobias; Neckernuss, Tobias; Mertens, Lina Katinka; Martin, Ines; Beil, Michael; Walther, Paul; Schimmel, Thomas; Marti, Othmar

Abstract (englisch):
Active microrheology is a valuable tool to determine viscoelastic properties of polymer networks. Observing the response of the beads to the excitation of a reference leads to dynamic and morphological information of the material. In this work we present an expansion of the well-known active two-point microrheology. By measuring the response of multiple particles in a viscoelastic medium in response to the excitation of a reference particle, we are able to determine the force propagation in the polymer network. For this purpose a lock-in technique is established that allows for extraction of the periodical motion of embedded beads. To exert a sinusoidal motion onto the reference bead an optical tweezers setup in combination with a microscope is used to investigate the motion of the response beads. From the lock-in data the so called transfer tensor can be calculated, which is a direct measure for the ability of the network to transmit mechanical forces. We also take a closer look at the influence of noise on lock-in measurements and state some simple rules for improving the signal-to-noise ratio.

Open Access Logo

Volltext §
DOI: 10.5445/IR/1000054550
DOI: 10.3762/bjnano.7.42
Zitationen: 1
Web of Science
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 2190-4286
KITopen-ID: 1000054550
HGF-Programm 43.22.01 (POF III, LK 01)
Functionality by Design
Erschienen in Beilstein Journal of Nanotechnology
Band 7
Seiten 484-491
Schlagwörter cytoskeleton, intermediate filaments, lock-in technique, microrheology, optical tweezers
Nachgewiesen in Scopus
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page