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Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development

Takamiya, M.; Xu, F.; Suhonen, H.; Gourain, V.; Yang, L.; Yu Ho, N.; Helfen, L.; Schröck, A.; Etard, C.; Grabher, C.; Rastegar, S.; Schlunck, G.; Reinhard, T.; Baumbach, T.; Strähle, U.



Abstract (englisch): Altered levels of trace elements are associated with increased oxidative stress that is eventually responsible for pathologic conditions. Oxidative stress has been proposed to be involved in eye diseases, including cataract formation. We visualized the distribution of metals and other trace elements in the eye of zebrafish embryos by micro X-ray fluorescence (μ-XRF) imaging. Many elements showed highest accumulation in the retinal pigment epithelium (RPE) of the zebrafish embryo. Knockdown of the zebrafish brown locus homologues tyrp1a/b eliminated accumulation of these elements in the RPE, indicating that they are bound by mature melanosomes. Furthermore, albino (slc45a2) mutants, which completely lack melanosomes, developed abnormal lens reflections similar to the congenital cataract caused by mutation of the myosin chaperon Unc45b, and an in situ spin trapping assay revealed increased oxidative stress in the lens of albino mutants. Finally transplanting a wildtype lens into an albino mutant background resulted in cataract formation. These data suggest that melanosomes in pigment epithelial cells protect the lens from oxidative stress during embryonic development, likely by buffering trace elements.


Zugehörige Institution(en) am KIT Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator DOI: 10.1038/srep25046
ISSN: 2045-2322
URN: urn:nbn:de:swb:90-546670
KITopen ID: 1000054667
HGF-Programm 47.01.01; LK 01
Erschienen in Scientific Reports
Band 6
Seiten 25046
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte Embryonic induction; Mechanisms of disease; X-ray tomography
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