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DOI: 10.5445/IR/1000054680

High-Throughput Process Development in the Field of Protein Purification - Method Development, Application, and Characterization

Diederich, Patrick

Abstract:
High-throughput methods were developed for the quantification of mAb aggregate/monomer ratios, and the analysis of content, purity, and activity of the egg white protein avidin. Furthermore, by using high-throughput screenings, a new avidin purification process was developed using precipitation, aqueous two-phase extraction and mixed-mode chromatography. Finally, method-specific effects present in high-throughput column chromatography were evaluated using both experimental and simulation data.


Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Hochschulschrift
Jahr 2015
Sprache Englisch
Identifikator URN: urn:nbn:de:swb:90-546805
KITopen ID: 1000054680
Verlag Karlsruhe
Umfang IX, 190 S.
Abschlussart Dissertation
Fakultät Fakultät für Chemieingenieurwesen und Verfahrenstechnik (CIW)
Institut Institut für Bio- und Lebensmitteltechnik (BLT)
Prüfungsdaten 15.06.2015
Referent/Betreuer Prof. J. Hubbuch
Schlagworte High-Throughput Process Development
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