High-Throughput Process Development in the Field of Protein Purification - Method Development, Application, and Characterization

High-throughput methods were developed for the quantification of mAb aggregate/monomer ratios, and the analysis of content, purity, and activity of the egg white protein avidin. Furthermore, by using high-throughput screenings, a new avidin purification process was developed using precipitation, aqueous two-phase extraction and mixed-mode chromatography. Finally, method-specific effects present in high-throughput column chromatography were evaluated using both experimental and simulation data.