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Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish

Frohnhöfer, Hans Georg; Geiger-Rudolph, Silke; Pattky, Martin; Meixner, Martin; Huhn, Carolin; Maischein, Hans-Martin; Geisler, Robert 1; Gehring, Ines; Maderspacher, Florian; Nüsslein-Volhard, Christiane; Irion, Uwe
1 Karlsruher Institut für Technologie (KIT)

Abstract:

Polyamines are small poly-cations essential for all cellular life. The main polyamines present in metazoans are putrescine, spermidine and spermine. Their exact functions are still largely unclear; however, they are involved in a wide variety of processes affecting cell growth, proliferation, apoptosis and aging. Here we identify idefix, a mutation in the zebrafish gene encoding the enzyme spermidine synthase, leading to a severe reduction in spermidine levels as shown by capillary electrophoresis-mass spectrometry. We show that spermidine, but not spermine, is essential for early development, organogenesis and colour pattern formation. Whereas in other vertebrates spermidine deficiency leads to very early embryonic lethality, maternally provided spermidine synthase in zebrafish is sufficient to rescue the early developmental defects. This allows us to uncouple them from events occurring later during colour patterning. Factors involved in the cellular interactions essential for colour patterning, likely targets for spermidine, are the gap junction components Cx41.8, Cx39.4, and Kir7.1, an inwardly rectifying potassium channel, all known to be regulated by polyamines. ... mehr


Volltext §
DOI: 10.5445/IR/1000055304
Originalveröffentlichung
DOI: 10.1242/bio.018721
Scopus
Zitationen: 32
Web of Science
Zitationen: 29
Dimensions
Zitationen: 34
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 15.06.2016
Sprache Englisch
Identifikator ISSN: 2046-6390
urn:nbn:de:swb:90-553047
KITopen-ID: 1000055304
HGF-Programm 47.01.04 (POF III, LK 01) Stock Centre
Erschienen in Biology open
Verlag The Company of Biologists
Band 5
Heft 6
Seiten 736-744
Vorab online veröffentlicht am 23.05.2016
Nachgewiesen in Dimensions
Scopus
Web of Science
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