KIT | KIT-Bibliothek | Impressum | Datenschutz

Sequential Mixed Cultures : From Syngas to Malic Acid

Oswald, Florian; Dörsam, Stefan; Veith, Nicolas; Zwick, Michaela; Neumann, Anke; Ochsenreither, Katrin; Syldatk, Christoph

Synthesis gas (syngas) fermentation using acetogenic bacteria is an approach for production of bulk chemicals like acetate, ethanol, butanol, or 2,3-butandiol avoiding the fuel vs. food debate by using carbon monoxide, carbon dioxide, and hydrogen from gasification of biomass or industrial waste gases. Suffering from energetic limitations, yields of C₄-molecules produced by syngas fermentation are quite low compared with ABE fermentation using sugars as a substrate. On the other hand, fungal production of malic acid has high yields of product per gram metabolized substrate but is currently limited to sugar containing substrates. In this study, it was possible to show that Aspergilus oryzae is able to produce malic acid using acetate as sole carbon source which is a main product of acetogenic syngas fermentation. Bioreactor cultivations were conducted in 2.5 L stirred tank reactors. During the syngas fermentation part of the sequential mixed culture, Clostridium ljungdahlii was grown in modified Tanner medium and sparged with 20 mL/min of artificial syngas mimicking a composition of clean syngas from entrained bed gasification of straw (32.5 vol-% CO, 32.5 vol-% H₂, 16 vol-% CO₂, and 19 vol-% N₂) using a microsparger. ... mehr

Open Access Logo

Volltext §
DOI: 10.5445/IR/1000055685
DOI: 10.3389/fmicb.2016.00891
Zitationen: 16
Web of Science
Zitationen: 14
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 1664-302X
KITopen-ID: 1000055685
HGF-Programm 35.13 (POF III, LK 01)
Biomasse für chemische Energieträger
Erschienen in Frontiers in microbiology
Band 7
Seiten 891
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagwörter syngas, fermentation, Clostridium ljungdahlii, Aspergillus oryzae, malic acid, acetate, process coupling
Nachgewiesen in Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page