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High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material

Loeffler, Felix F.; Foertsch, Tobias C.; Popov, Roman; Mattes, Daniela S.; Schlageter, Martin; Sedlmayr, Martyna; Ridder, Barbara; Dang, Florian-Xuan; Bojnicic-Kninski, Clemens von; Weber, Laura K.; Fischer, Andrea; Greifenstein, Juliane; Bykovskaya, Valentina; Buliev, Ivan; Bischoff, F. Ralf; Hahn, Lothar; Meier, Michael A.R.; Bräse, Stefan; Powell, Annie K.; Balaban, Teodor Silviu; ... mehr

Abstract (englisch):
Laser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic monomers. A laser automatically transfers nanometre-thin solid material spots from different donor slides to an acceptor. Each donor bears a thin polymer film, embedding one type of monomer. Coupling occurs in a separate heating step, where the matrix becomes viscous and building blocks diffuse and couple to the acceptor surface. Furthermore, we can consecutively deposit two material layers of activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in situ activation and coupling of the monomers. This allows us to incorporate building blocks with click chemistry compatibility or a large variety of commercially available non-activated, for example, posttranslationally modified building blocks into the array’s peptides with >17,000 spots per cm².


Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Institut für Nanotechnologie (INT)
Institut für Organische Chemie (IOC)
Institut für Toxikologie und Genetik (ITG)
Institut für Anorganische Chemie (AOC)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator DOI: 10.1038/ncomms11844
ISSN: 2041-1723
URN: urn:nbn:de:swb:90-556923
KITopen ID: 1000055692
HGF-Programm 47.01.03; LK 01
Erschienen in Nature Communications
Band 7
Seiten Article number 11844
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte Chemical sciences; Materials science; Organic chemistry; Chemical biology
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