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Toxicity testing of combustion aerosols at the air-liquid interface with a self-contained and easy-to-use exposure system

Mülhopt, Sonja ORCID iD icon 1; Dilger, Marco 2; Diabate, Silvia 2; Schlager, Christoph 1; Krebs, Tobias; Zimmermann, Ralf; Buters, Jeroen; Oeder, Sebastian; Waescher, Thomas; Weiss, Carsten 2; Paur, Hanns-Rudolf 3
1 Institut für Technische Chemie (ITC), Karlsruher Institut für Technologie (KIT)
2 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)
3 Karlsruher Institut für Technologie (KIT)


In vitro toxicity testing of airborne particles usually takes place in multi-well plates, where the cells are exposed to a suspension of particles in cell culture medium. Due to the artefacts caused by particle collection and preparation of suspensions, the air–liquid interface (ALI) exposure is challenging this conventional exposure technique to become the method of choice. The ALI technique allows for direct sampling of an aerosol and exposure of cell cultures to airborne particles. At the same time, it reflects the physiological conditions in the lung to a greater extent. So far, the available ALI systems have mostly been laboratory set-ups of the single components. Here, we present a mobile and complete system providing all process technology required for cell exposure experiments at dynamic aerosol sources. The system is controlled by a human machine interface (HMI) with standard routines for experiments and internal testing to assure reproducibility. It also provides documentation of the exposure experiment regarding process parameters and measured doses. The performance of this system is evaluated using fluorescein-sodium dosimetry, which is also used to determine the factor of dose enhancement by optional electrostatic deposition. ... mehr

DOI: 10.1016/j.jaerosci.2016.02.005
Zitationen: 54
Web of Science
Zitationen: 50
Zitationen: 58
Zugehörige Institution(en) am KIT Institut für Technische Chemie (ITC)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 0021-8502
KITopen-ID: 1000055710
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Journal of Aerosol Science
Verlag Elsevier
Band 96
Seiten 38-55
Vorab online veröffentlicht am 09.03.2016
Schlagwörter Air–liquid interface exposure, Nanoparticle, Lung cell culture, In-vitro, Ship diesel emission, Wood combustion
Nachgewiesen in Dimensions
Web of Science
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