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Involvement of the cohesin cofactor PDS5 (SPO76) during meiosis and DNA repair in Arabidopsis thaliana

Pradillo, M.; Knoll, A.; Oliver, C.; Varas, J.; Corredor, E.; Puchta, H.; Santos, J.L.

Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cellcycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DANN repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes(A–E) and obtained, after different crosses between them, double,triple,and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DANN repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to ist function in other species, our results sugges ... mehr

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DOI: 10.5445/IR/1000056800
DOI: 10.3389/fpls.2015.01034
Zitationen: 10
Web of Science
Zitationen: 8
Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 1664-462X
KITopen-ID: 1000056800
Erschienen in Frontiers in plant science
Band 6
Heft DEC
Seiten Article Nr. 1034
Schlagworte Arabidopsis thaliana, cohesin cofactor, DNA repair ,homologous recombination, Meiosis, PDS5, SPO76
Nachgewiesen in Web of Science
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