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Response to gaseous NO₂ air pollutant of P. fluorescens airborne strain MFAF76a and clinical strain MFN1032

Kondakova, Tatiana; Catovic, Chloe; Barreau, Magalie; Nusser, Michael; Brenner-Weiss, Gerald; Chevalier, Sylvie; Dionnet, Frederic; Orange, Nicole; Poc, Cecile Duclairoir

Abstract (englisch):
Human exposure to nitrogen dioxide (NO2), an air pollutant of increasing interest in biology, results in several toxic effects to human health and also to the air microbiota. The aim of this study was to investigate the bacterial response to gaseous NO2. Two Pseudomonas fluorescens strains, namely the airborne strain MFAF76a and the clinical strain MFN1032 were exposed to 0.1, 5, or 45 ppm concentrations of NO2, and their effects on bacteria were evaluated in terms of motility, biofilm formation, antibiotic resistance, as well as expression of several chosen target genes. While 0.1 and 5 ppm of NO2did not lead to any detectable modification in the studied phenotypes of the two bacteria, several alterations were observed when the bacteria were exposed to 45 ppm of gaseous NO2. We thus chose to focus on this high concentration. NO2-exposed P. fluorescens strains showed reduced swimming motility, and decreased swarming in case of the strain MFN1032. Biofilm formed by NO2-treated airborne strain MFAF76a showed increased maximum thickness compared to non-treated cells, while NO2 had no apparent effect on the clinical MFN1032 biofilm structure. ... mehr

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Volltext §
DOI: 10.5445/IR/1000057486
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator ISSN: 1664-302X
KITopen-ID: 1000057486
HGF-Programm 47.02.06 (POF III, LK 01)
Erschienen in Frontiers in microbiology
Band 7
Seiten Art.Nr.: 379
Nachgewiesen in Scopus
Web of Science
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