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URN: urn:nbn:de:swb:90-579944
DOI: 10.1242/bio.201410892
Web of Science
Zitationen: 7

RHAMM deficiency disrupts folliculogenesis resulting in female hypofertility

Li, Huaibiao; Moll, Juergen; Winkler, Anne; Frappart, Lucien; Brunet, Phane; Hamann, Jana; Kroll, Torsten; Verlhac, Marie-Helene; Heuer, Heike; Herrlich, Peter; Ploubidou, Aspasia

The postnatal mammalian ovary contains the primary follicles, each comprising an immature oocyte surrounded by a layer of somatic granulosa cells. Oocytes reach meiotic and developmental competence via folliculogenesis. During this process, the granulosa cells proliferate massively around the oocyte, form an extensive extracellular matrix (ECM) and differentiate into cumulus cells. As the ECM component hyaluronic acid (HA) is thought to form the backbone of the oocyte-granulosa cell complex, we deleted the relevant domain of the Receptor for HA Mediated Motility (RHAMM) gene in the mouse. This resulted in folliculogenesis defects and female hypofertility, although HA-induced signalling was not affected. We report that wild-type RHAMM localises at the mitotic spindle of granulosa cells, surrounding the oocyte. Deletion of the RHAMM C-terminus in vivo abolishes its spindle association, resulting in impaired spindle orientation in the dividing granulosa cells, folliculogenesis defects and subsequent female hypofertility. These data reveal the first identified physiological function for RHAMM, during oogenesis, and the importance of thi ... mehr

Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 2046-6390
KITopen ID: 1000057994
HGF-Programm 47.01.01; LK 01
Erschienen in Biology open
Band 4
Heft 4
Seiten 562-571
Schlagworte Gametogenesis, Folliculogenesis, Hypofertility, Spindle, RHAMM, Oriented mitosis, Centrosome
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