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Regulation of distinct branches of the non-canonical Wnt-signaling network in Xenopus dorsal marginal zone explants

Wallkamm, Veronika; Rahm, Karolin; Schmoll, Jana; Kaufmann,Lilian T.; Brinkmann, Eva; Schunk, Jessica; Kraft, Bianca; Wedlich, Doris; Gradl, Dietmar

Background: A tight regulation of the Wnt-signaling network, activated by 19 Wnt molecules and numerous receptors and co-receptors, is required for the establishment of a complex organism. Different branches of this Wnt-signaling network, including the canonical Wnt/β-catenin and the non-canonical Wnt/PCP, Wnt/Ror2 and Wnt/Ca$^{2+}$ pathways, are assigned to distinct developmental processes and are triggered by certain ligand/receptor complexes. The Wnt-signaling molecules are closely related and it is still on debate whether the information for activating a specific branch is encoded by specific sequence motifs within a particular Wnt protein. The model organism Xenopus offers tools to distinguish between Wnt-signaling molecules activating distinct branches of the network.
Results: We created chimeric Wnt8a/Wnt11 molecules and could demonstrate that the C-terminal part (containing
the BS2) of Wnt8a is responsible for secondary axis formation. Chimeric Wnt11/Wnt5a molecules revealed that the
N-terminus with the elements PS3-1 and PS3-2 defines Wnt11 specificity, while elements PS3-1, PS3-2 and PS3-3 are
required for Wnt5a specificity. ... mehr

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Volltext §
DOI: 10.5445/IR/1000058176
DOI: 10.1186/s12915-016-0278-x
Zitationen: 2
Web of Science
Zitationen: 2
Zugehörige Institution(en) am KIT Zoologisches Institut (ZOO)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator ISSN: 1741-7007
KITopen-ID: 1000058176
Erschienen in BMC biology
Band 14
Heft 55
Seiten 201614
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte Wnt-signaling network, convergent extension movements, non-canonical Wnt-signaling
Nachgewiesen in Web of Science
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